Allis (Alosa alosa) and Twaite (Alosa fallax) shad monitoring data sampled from the rivers Wye, Usk and Tywi, using Mitochondrial DNA techniques (2014).
Populations of allis shad (Alosa alosa Lacépède) and twaite shad (Alosa fallax L.) have declined across their geographic distribution over the last century and despite being widespread around the coast of the British Isles, UK spawning populations are largely restricted to the Rivers Severn, Wye, Usk and Tywi which drain into the Bristol Channel. As a result, both species are protected by UK and international legislation. In response to a need to monitor the population status to inform conservation objectives for these species, the former Countryside Council for Wales and Environment Agency Wales, now Natural Resources Wales (NRW) developed a semi-quantitative and cost-effective method to assess recruitment potential by sampling shad eggs. However, this method is unable to distinguish between the two Alosa species, and there is a risk that eggs of other fish species may erroneously be recorded as shad eggs. The aim of this data collection was to use genetic techniques to address the weaknesses related to the semi-quantitative and cost-effective methods adopted being unable to distinguish between the two Alosa species, and the risks that other eggs related to other fish species being erroneously recorded. The two objectives were to: (a) Determine whether the collected eggs belonged to Alosa spp. (b) Determine the proportion of A. fallax and A. alosa using three different genetic markers – mitochondrial DNA and two types of nuclear DNA: nif1-nDNA and six microsatellite loci. A total of 226 eggs were successfully genotyped. 85% belonged to Alosa spp. demonstrating that a good level of egg identification in the collection team. Non-Alosa eggs were identified as belonging to minnow (Phoxinus phoxinus) and chub (Squalius cephalus). As a result, the extent of putative shad spawning on the Tywi is slightly less than suggested by the egg surveys alone. However, the upper extent of shad spawning on the Wye was confirmed as being Brynwern Bridge, about 9km further upstream than the previous known upper limit at Builth Wells. Methods of data capture:
- Identification
- Extent
- Distribution
- Quality
- Keywords
- Spatial Reference System
- Content
- Constraints
- Maintenance
- Metadata
Identification
- Identifier
- NRW_DS115891
- Alternative Title
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- Data Herlynod (Alosa alosa) a Gwangod (Alosa fallax) a samplwyd yn afonydd Gwy, Wysg a Thywi, gan ddefnyddio technegau DNA Mitocondriaidd (2014)
- Metadata Language
- English
- Lineage
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Staff were issued with clear instructions and standard field equipment including pre-labelled 1.5 ml Eppendorf tubes pre-loaded with buffer solution and coolboxes. Up to 20 eggs were collected from each sampling site and placed in ATL (lysing) buffer in pre-labelled 1.5ml Eppendorf tubes (Table 1). Eggs were either immediately couriered by overnight service to the laboratory, or else refrigerated overnight and couriered the following day. During the spawning season (June 2013), Natural Resources Wales (NRW) staff collected 240 individual eggs by kick sampling from 12 sampling sites in the rivers Tywi, Usk and Wye. Two mitochondrial (mtDNA) haplogroups for the cytochrome B (CytB) gene have been previously identified in Alosa spp.; haplogroup A and haplogroup F are mainly associated with A. alosa and A. fallax respectively. Using the primers described in Alexandrino et al. (2006), a fragment of 401 bp of the (CytB) was successfully amplified. Out of the 226 eggs extracted, 191 samples were amplified using CytB specific primers.
- Additional Information
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Monitoring Allis and Twaite Shad: Quality assurance and species Identification using molecular techniques. Dr E Hardouin, Dr D Andreou and S Stuart Bournemouth University
- Dataset Reference Date (Publication)
- 2015-05-31
Extent
Extent
- Geographic Extent
- SIR FYNWY - MONMOUTHSHIRE
- Geographic Extent
- BLAENAU GWENT - BLAENAU GWENT
- Geographic Extent
- MERTHYR TUDFUL - MERTHYR TYDFIL
- Geographic Extent
- TOR-FAEN - TORFAEN
- Geographic Extent
- CASNEWYDD - NEWPORT
- Geographic Extent
- CAERFFILI - CAERPHILL
- Geographic Extent
- CAERDYDD - CARDIFF
- Geographic Extent
- BRO MORGANNWG - THE VALE OF GLAMORGAN
- Geographic Extent
- PEN-Y-BONT AR OGWR - BRIDGEND
- Geographic Extent
- CASTELL-NEDD PORT TALBOT - NEATH PORT TALBOT
- Geographic Extent
- ABERTAWE - SWANSEA
- Geographic Extent
- SIR GAERFYRDDIN - CARMARTHENSHIRE
- Geographic Extent
- SIR BENFRO - PEMBROKESHIRE
- Geographic Extent
- SIR CEREDIGION - CEREDIGION
- Geographic Extent
- POWYS - POWYS
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Distribution
- Format Type and Description
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Documents
()
- Specification
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The data archive contains: [A] The final report in Microsoft Word and Adobe PDF formats. [B] Electronic copies of the field sheets, saved as pdfs. [C] An Excel spreadsheet Ninf_mito_microsat_scoring (NRW-13-066287) detailing the RFLP, mtDNA and microsatellite results for each egg. [D] Three FASTA format files, TreeUniveralPrimers2.fas (NRW-13-084552); Ninf_sequences.fas (NRW-13-066284) and Shad_CytochromeB_all2.fas (NRW-13-066283).
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Documents
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Quality
Data quality
- Quality Scope
- Dataset
- Lineage
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Staff were issued with clear instructions and standard field equipment including pre-labelled 1.5 ml Eppendorf tubes pre-loaded with buffer solution and coolboxes. Up to 20 eggs were collected from each sampling site and placed in ATL (lysing) buffer in pre-labelled 1.5ml Eppendorf tubes (Table 1). Eggs were either immediately couriered by overnight service to the laboratory, or else refrigerated overnight and couriered the following day. During the spawning season (June 2013), Natural Resources Wales (NRW) staff collected 240 individual eggs by kick sampling from 12 sampling sites in the rivers Tywi, Usk and Wye. Two mitochondrial (mtDNA) haplogroups for the cytochrome B (CytB) gene have been previously identified in Alosa spp.; haplogroup A and haplogroup F are mainly associated with A. alosa and A. fallax respectively. Using the primers described in Alexandrino et al. (2006), a fragment of 401 bp of the (CytB) was successfully amplified. Out of the 226 eggs extracted, 191 samples were amplified using CytB specific primers.
Keywords
Keywords
- Type
- Theme
Citation
- Date (Publication)
- 2008-06-01
Keywords
- NRW Thesaurus
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- River Usk
- River Wye (Afon Gwy)
- twaite shad
- alosa fallax
- allis shad
- alosa alosa
- Shad
- biological monitoring
- biological surveys
- Type
- Theme
Citation
- Date (Publication)
- 2023-12-31
Spatial Reference System
Content
Content Information
NRW Profile
Custom Elements
- NRW Related Title
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Monitoring allis and twaite shad : quality assurance and species identification using molecular techniques
Constraints
Constraints
Limitations on Public Access and Use
- Restriction type
- Other restrictions
Access Constraints Directive
- Limitations
- no limitations
Access Constraints Text
- Other constraints
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There are no access restrictions to this data. NRW may release, publish or disseminate it freely.
Use Constraints
- Use constraints type
- Other restrictions
- Other constraints
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© CNC/NRW, 2015. There are no use restrictions on this data. Recipients may re-use, reproduce, disseminate this data free of charge in any format or medium, provided they do so accurately, acknowledging both the source and NRW's copyright, and do not use it in a misleading context. It is the recipient's responsibility to ensure the data is fit for the intended purpose, that dissemination or publishing does not result in duplication, and that it is fairly interpreted. Advice on interpretation should be sought where required. To avoid re-using old data, users should periodically obtain the latest version from the original source.
Metadata
Metadata
- File Identifier
- 4f4c4942-4343-5764-6473-313135383931 XML
- Metadata Language
- English
- Resource type
- Dataset
- Metadata Date
- 2024-05-31T09:31:41.928Z
- Metadata Standard Name
- NRW
- Metadata Standard Version
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1.0