4f4c4942-4343-5764-6473-313135383931 English Data Services Team Natural Resources Wales (NRW), Maes y Ffynnon, Penrhosgarnedd, Bangor, Gwynedd, LL57 2DW enquiries@naturalresourceswales.gov.uk http://naturalresourceswales.gov.uk/splash?orig=/ 2024-05-31T09:31:41.928Z NRW 1.0 wel British National Grid (EPSG::27700) Allis (Alosa alosa) and Twaite (Alosa fallax) shad monitoring data sampled from the rivers Wye, Usk and Tywi, using Mitochondrial DNA techniques (2014). Allis (Alosa alosa) and Twaite (Alosa fallax) shad monitoring data sampled from the rivers Wye, Usk and Tywi, using Mitochondrial DNA techniques (2014). Data Herlynod (Alosa alosa) a Gwangod (Alosa fallax) a samplwyd yn afonydd Gwy, Wysg a Thywi, gan ddefnyddio technegau DNA Mitocondriaidd (2014) 2015-05-31 NRW_DS115891 Populations of allis shad (Alosa alosa Lacépède) and twaite shad (Alosa fallax L.) have declined across their geographic distribution over the last century and despite being widespread around the coast of the British Isles, UK spawning populations are largely restricted to the Rivers Severn, Wye, Usk and Tywi which drain into the Bristol Channel. As a result, both species are protected by UK and international legislation. In response to a need to monitor the population status to inform conservation objectives for these species, the former Countryside Council for Wales and Environment Agency Wales, now Natural Resources Wales (NRW) developed a semi-quantitative and cost-effective method to assess recruitment potential by sampling shad eggs. However, this method is unable to distinguish between the two Alosa species, and there is a risk that eggs of other fish species may erroneously be recorded as shad eggs. The aim of this data collection was to use genetic techniques to address the weaknesses related to the semi-quantitative and cost-effective methods adopted being unable to distinguish between the two Alosa species, and the risks that other eggs related to other fish species being erroneously recorded. The two objectives were to: (a) Determine whether the collected eggs belonged to Alosa spp. (b) Determine the proportion of A. fallax and A. alosa using three different genetic markers – mitochondrial DNA and two types of nuclear DNA: nif1-nDNA and six microsatellite loci. A total of 226 eggs were successfully genotyped. 85% belonged to Alosa spp. demonstrating that a good level of egg identification in the collection team. Non-Alosa eggs were identified as belonging to minnow (Phoxinus phoxinus) and chub (Squalius cephalus). As a result, the extent of putative shad spawning on the Tywi is slightly less than suggested by the egg surveys alone. However, the upper extent of shad spawning on the Wye was confirmed as being Brynwern Bridge, about 9km further upstream than the previous known upper limit at Builth Wells. Methods of data capture: Populations of allis shad (Alosa alosa Lacépède) and twaite shad (Alosa fallax L.) have declined across their geographic distribution over the last century and despite being widespread around the coast of the British Isles, UK spawning populations are largely restricted to the Rivers Severn, Wye, Usk and Tywi which drain into the Bristol Channel. As a result, both species are protected by UK and international legislation. In response to a need to monitor the population status to inform conservation objectives for these species, the former Countryside Council for Wales and Environment Agency Wales, now Natural Resources Wales (NRW) developed a semi-quantitative and cost-effective method to assess recruitment potential by sampling shad eggs. However, this method is unable to distinguish between the two Alosa species, and there is a risk that eggs of other fish species may erroneously be recorded as shad eggs. The aim of this data collection was to use genetic techniques to address the weaknesses related to the semi-quantitative and cost-effective methods adopted being unable to distinguish between the two Alosa species, and the risks that other eggs related to other fish species being erroneously recorded. The two objectives were to: (a) Determine whether the collected eggs belonged to Alosa spp. (b) Determine the proportion of A. fallax and A. alosa using three different genetic markers – mitochondrial DNA and two types of nuclear DNA: nif1-nDNA and six microsatellite loci. A total of 226 eggs were successfully genotyped. 85% belonged to Alosa spp. demonstrating that a good level of egg identification in the collection team. Non-Alosa eggs were identified as belonging to minnow (Phoxinus phoxinus) and chub (Squalius cephalus). As a result, the extent of putative shad spawning on the Tywi is slightly less than suggested by the egg surveys alone. However, the upper extent of shad spawning on the Wye was confirmed as being Brynwern Bridge, about 9km further upstream than the previous known upper limit at Builth Wells. Methods of data capture: Natural Resources Wales (NRW) Maes-y-Ffynnon, Penrhosgarnedd, Bangor, Gwynedd, LL57 2DW, Wales enquiries@naturalresourceswales.gov.uk http://naturalresources.wales/?lang=en Natural Resources Wales (NRW) Maes-y-Ffynnon, Penrhosgarnedd, Bangor, Gwynedd, LL57 2DW, Wales enquiries@naturalresourceswales.gov.uk http://naturalresources.wales/?lang=en Natural Resources Wales (NRW) Maes-y-Ffynnon, Penrhosgarnedd, Bangor, Gwynedd, LL57 2DW, Wales enquiries@naturalresourceswales.gov.uk http://naturalresources.wales/?lang=en Bournemouth University Bournemouth University, Fern Barrow, Talbot Campus, Poole, Dorset, BH12 5BB, UK enquiries@bournemouth.ac.uk https://www1.bournemouth.ac.uk/ Natural Resources Wales (NRW) Maes-y-Ffynnon, Penrhosgarnedd, Bangor, Gwynedd, LL57 2DW, Wales enquiries@naturalresourceswales.gov.uk http://naturalresources.wales/?lang=en Habitats and biotopes GEMET - INSPIRE themes, version 1.0 GEMET - INSPIRE themes, version 1.0 2008-06-01 geonetwork.thesaurus.external.theme.httpinspireeceuropaeutheme-theme River Usk River Wye (Afon Gwy) twaite shad alosa fallax allis shad alosa alosa Shad biological monitoring biological surveys NRW Thesaurus NRW Thesaurus 2023-12-31 geonetwork.thesaurus.local.theme.converted_nrw-keywords There are no access restrictions to this data. NRW may release, publish or disseminate it freely. no limitations © CNC/NRW, 2015. There are no use restrictions on this data. Recipients may re-use, reproduce, disseminate this data free of charge in any format or medium, provided they do so accurately, acknowledging both the source and NRW's copyright, and do not use it in a misleading context. It is the recipient's responsibility to ensure the data is fit for the intended purpose, that dissemination or publishing does not result in duplication, and that it is fairly interpreted. Advice on interpretation should be sought where required. To avoid re-using old data, users should periodically obtain the latest version from the original source. English biota SeaVoX Vertical Co-ordinate Coverages SeaVoX Vertical Co-ordinate Coverages 2021-01-06 SIR FYNWY - MONMOUTHSHIRE BLAENAU GWENT - BLAENAU GWENT MERTHYR TUDFUL - MERTHYR TYDFIL TOR-FAEN - TORFAEN CASNEWYDD - NEWPORT CAERFFILI - CAERPHILL CAERDYDD - CARDIFF BRO MORGANNWG - THE VALE OF GLAMORGAN PEN-Y-BONT AR OGWR - BRIDGEND CASTELL-NEDD PORT TALBOT - NEATH PORT TALBOT ABERTAWE - SWANSEA SIR GAERFYRDDIN - CARMARTHENSHIRE SIR BENFRO - PEMBROKESHIRE SIR CEREDIGION - CEREDIGION POWYS - POWYS -5.56369856 -2.6827358 51.28073225 52.51490863 2013-06-10 2013-06-13 Monitoring Allis and Twaite Shad: Quality assurance and species Identification using molecular techniques. Dr E Hardouin, Dr D Andreou and S Stuart Bournemouth University Monitoring allis and twaite shad : quality assurance and species identification using molecular techniques Documents The data archive contains: [A] The final report in Microsoft Word and Adobe PDF formats. [B] Electronic copies of the field sheets, saved as pdfs. [C] An Excel spreadsheet Ninf_mito_microsat_scoring (NRW-13-066287) detailing the RFLP, mtDNA and microsatellite results for each egg. [D] Three FASTA format files, TreeUniveralPrimers2.fas (NRW-13-084552); Ninf_sequences.fas (NRW-13-066284) and Shad_CytochromeB_all2.fas (NRW-13-066283). Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 false Staff were issued with clear instructions and standard field equipment including pre-labelled 1.5 ml Eppendorf tubes pre-loaded with buffer solution and coolboxes. Up to 20 eggs were collected from each sampling site and placed in ATL (lysing) buffer in pre-labelled 1.5ml Eppendorf tubes (Table 1). Eggs were either immediately couriered by overnight service to the laboratory, or else refrigerated overnight and couriered the following day. During the spawning season (June 2013), Natural Resources Wales (NRW) staff collected 240 individual eggs by kick sampling from 12 sampling sites in the rivers Tywi, Usk and Wye. Two mitochondrial (mtDNA) haplogroups for the cytochrome B (CytB) gene have been previously identified in Alosa spp.; haplogroup A and haplogroup F are mainly associated with A. alosa and A. fallax respectively. Using the primers described in Alexandrino et al. (2006), a fragment of 401 bp of the (CytB) was successfully amplified. Out of the 226 eggs extracted, 191 samples were amplified using CytB specific primers. Staff were issued with clear instructions and standard field equipment including pre-labelled 1.5 ml Eppendorf tubes pre-loaded with buffer solution and coolboxes. Up to 20 eggs were collected from each sampling site and placed in ATL (lysing) buffer in pre-labelled 1.5ml Eppendorf tubes (Table 1). Eggs were either immediately couriered by overnight service to the laboratory, or else refrigerated overnight and couriered the following day. During the spawning season (June 2013), Natural Resources Wales (NRW) staff collected 240 individual eggs by kick sampling from 12 sampling sites in the rivers Tywi, Usk and Wye. Two mitochondrial (mtDNA) haplogroups for the cytochrome B (CytB) gene have been previously identified in Alosa spp.; haplogroup A and haplogroup F are mainly associated with A. alosa and A. fallax respectively. Using the primers described in Alexandrino et al. (2006), a fragment of 401 bp of the (CytB) was successfully amplified. Out of the 226 eggs extracted, 191 samples were amplified using CytB specific primers.